Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Midnolin (ER1913-02) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD| Product Name | Midnolin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MIDN_HUMAN 3000003C15Rik DKFZp547M072 MIDN midnolin Midbrain nucleolar protein. |
| Product description | Expression of the midnolin gene is developmentally regulated: it is strongly expressed at the mesencephalon (midbrain) of the embryo in day 12.5 (E12.5) mice. The midnolin encodes a protein of 508 amino acids (aa); which contains a Ubiquitin-like domain. The intracellular distribution of the midnolin was studied by using midnolin-green fluorescent protein (GFP) fusion proteins. Midnolin was found to be localized in the nucleus and nucleolus; but not in the cytoplasm. The nucleolar localization sigl was determined to be a 28aa peptide located at the C-termil region of the midnolin. May be involved in regulation of genes related to neurogenesis in the nucleolus |
| Immunogen | KLH conjugated synthetic peptide derived from human MIDN 51-150/468 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowGuinea PigOther |
| Concentration | 1mg/ml |
| Alternative Names | MIDN_HUMAN 3000003C15Rik DKFZp547M072 MIDN midnolin Midbrain nucleolar protein. |
|---|---|
| Molecular Weight(MW) | 49 KDa |
| Cellular Localization | Nucleus, nucleolus. |
| SwissProt ID | Q504T8 |
|---|
Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Midnolin (ER1913-02) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD| Positive Control | Mouse Liver Lysate, Mouse Cerebrum Lysate, rat brain tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
