Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-MNAT1 (ER1913-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD| Product Name | MNAT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MNAT 1 CAP35 CDK activating kinase assembly factor MAT1 CDK7/cyclin H assembly factor Cyclin G1 interacting protein MAT1 Menage a trois MNAT 1 p35 p36 RING finger protein 66 RING finger protein MAT1 RNF66 MAT1_HUMAN. |
| Product description | Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. |
| Immunogen | KLH conjugated synthetic peptide derived from human MNAT1 51-150/309 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | MNAT 1 CAP35 CDK activating kinase assembly factor MAT1 CDK7/cyclin H assembly factor Cyclin G1 interacting protein MAT1 Menage a trois MNAT 1 p35 p36 RING finger protein 66 RING finger protein MAT1 RNF66 MAT1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P51948 |
|---|
Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-MNAT1 (ER1913-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD| Positive Control | Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
