Application
Fig1: Positive control: H9C2
Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μg in 100 μL1X PBS containing 0.5% BSA.
Application
Fig2: Blank control: H9C2(blue)
Isotype Control Antibody: Rabbit IgG -FITC(orange); Primary Antibody Dilution: 12μl in 100 μL1X PBS containing 0.5% BSA(green).
Application
Fig3: Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- MOT8 (ER1913-12) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD