Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MRF/C11orf9 Polyclonal Antibody, Unconjugated(ER1913-17) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-MRF/C11orf9 (ER1913-17) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 124 kD Observed band size: 124 kDApplication
Fig3: Sample: Uterus (Mouse) Lysate at 40 ug Primary: Anti- MRF/C11orf9 (ER1913-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kDApplication
Fig4: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti- MRF/C11orf9 (ER1913-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kDApplication
Fig5: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- MRF/C11orf9 (ER1913-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD| Product Name | MRF/C11orf9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C11orf9 Chromosome 11 open reading frame 9 Gm1804 gm98 KIAA0954 MGC10781 MRF MRF_HUMAN Myelin gene regulatory factor Ndt80. |
| Product description | Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes. |
| Immunogen | KLH conjugated synthetic peptide derived from human MRF 401-500/1151 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | C11orf9 Chromosome 11 open reading frame 9 Gm1804 gm98 KIAA0954 MGC10781 MRF MRF_HUMAN Myelin gene regulatory factor Ndt80. |
|---|---|
| Molecular Weight(MW) | 124 KDa |
| Cellular Localization | Membrane. Nucleus. |
| SwissProt ID | Q9Y2G1 |
|---|
Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MRF/C11orf9 Polyclonal Antibody, Unconjugated(ER1913-17) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-MRF/C11orf9 (ER1913-17) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 124 kD Observed band size: 124 kD
Application
Fig3: Sample: Uterus (Mouse) Lysate at 40 ug Primary: Anti- MRF/C11orf9 (ER1913-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD
Application
Fig4: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti- MRF/C11orf9 (ER1913-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD
Application
Fig5: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- MRF/C11orf9 (ER1913-17) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD| Positive Control | Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
