Application
Fig1: Sample: Rectum (Mouse) Lysate at 40 ug Primary: Anti- MTM1 (ER1913-27) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 68 kD| Product Name | MTM1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CG2 CNM KIAA4176 mKIAA4176 Mtm Mtm1 MTM1_HUMAN MTMX Myotubular myopathy 1 Myotubularin XLMTM. |
| Product description | Lipid phosphatase which dephosphorylates phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Has also been shown to dephosphorylate phosphotyrosine- and phosphoserine-containing peptides. Negatively regulates EGFR degradation through regulation of EGFR trafficking from the late endosome to the lysosome. Plays a role in vacuolar formation and morphology. Regulates desmin intermediate filament assembly and architecture. Plays a role in mitochondrial morphology and positioning. Required for skeletal muscle maintenance but not for myogenesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human MTM1/Myotubularin 201-300/603 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CG2 CNM KIAA4176 mKIAA4176 Mtm Mtm1 MTM1_HUMAN MTMX Myotubular myopathy 1 Myotubularin XLMTM. |
|---|---|
| Molecular Weight(MW) | 70 KDa |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Late endosome. Note=Localizes as a dense cytoplasmic network. Also localizes to the plasma membrane, including plasma membrane extensions such as filopodia and ruffles. Predominantly located in the cytoplasm following interaction with MTMR12. Recruited to the late endosome following EGF stimulation. [DOMAIN] The GRAM domain mediates binding to PI(3,5)P2 and, with lower affinity, to other phosphoinositides. |
| SwissProt ID | Q13496 |
|---|
Application
Fig1: Sample: Rectum (Mouse) Lysate at 40 ug Primary: Anti- MTM1 (ER1913-27) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 68 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
