Application
Fig1: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-MT-ND5 (ER1913-30) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kDApplication
genous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MT-ND5 Polyclonal Antibody, Unconjugated(ER1913-30) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: rat brain tissue 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MT-ND5 Polyclonal Antibody, Unconjugated(ER1913-30) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MT-ND5) Polyclonal Antibody, Unconjugated (ER1913-30) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | MT-ND5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Complex I, subunit ND5 Mitochondrially encoded NADH dehydrogenase 5 MT ND5 MTND 5 MTND5 NAD5 NADH dehydrogenase subunit 5 (complex I) NADH dehydrogenase subunit 5 NADH ubiquinone oxidoreductase , subunit ND5 NADH ubiquinone oxidoreductase chain 5 NADH5 ND5 EC 1.6.5.3.NU5M_HUMAN |
| Product description | NADH-ubiquinone oxidoreductase chain 5 is a protein that in humans is encoded by the mitochondrial gene MT-ND5. The ND5 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variations in MT-ND5 are associated with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) as well as some symptoms of Leigh's syndrome and Leber's hereditary optic neuropathy (LHON). MT-ND5 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix. |
| Immunogen | KLH conjugated synthetic peptide derived from human MT-ND5 451-550/603 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | Complex I subunit ND5 Mitochondrially encoded NADH dehydrogenase 5 MT ND5 MTND 5 MTND5 NAD5 NADH dehydrogenase subunit 5 (complex I) NADH dehydrogenase subunit 5 NADH ubiquinone oxidoreductase subunit ND5 NADH ubiquinone oxidoreductase chain 5 NADH5 ND5 EC 1.6.5.3.NU5M_HUMAN |
|---|---|
| Molecular Weight(MW) | 67 KDa |
| Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein. |
| SwissProt ID | P03915 |
|---|
Application
Fig1: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-MT-ND5 (ER1913-30) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD
Application
genous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MT-ND5 Polyclonal Antibody, Unconjugated(ER1913-30) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: rat brain tissue 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MT-ND5 Polyclonal Antibody, Unconjugated(ER1913-30) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MT-ND5) Polyclonal Antibody, Unconjugated (ER1913-30) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Cerebrum (Rat) Lysate, human lung carcinoma, rat brain tissue, human liver carcinoma. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
