Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MTRF1) Polyclonal Antibody, Unconjugated (ER1913-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-MTRF1 (ER1913-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 43 kD| Product Name | MTRF1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HMRF1L Human mitochondrial release factor 1-like Mitochondrial release factor 1 like Mitochondrial translational release factor 1 like Mitochondrial translational release factor 1-like mtRF1a MTRF1L Peptide chain release factor 1-like, mitochondrial RF1ML_HUMAN. |
| Product description | Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG. |
| Immunogen | KLH conjugated synthetic peptide derived from human MTRF1 301-380/380 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | HMRF1L
Human mitochondrial release factor 1-like
Mitochondrial release factor 1 like
Mitochondrial translational release factor 1 like
Mitochondrial translational release factor 1-like
mtRF1a
MTRF1L
Peptide chain release factor 1-like mitochondrial RF1ML_HUMAN. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Mitochondrion. |
| SwissProt ID | Q9UGC7 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MTRF1) Polyclonal Antibody, Unconjugated (ER1913-33) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-MTRF1 (ER1913-33) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 43 kD| Positive Control | Expressed in skeletal muscle (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
