Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-muscarinic Acetylcholine Receptor 1 (ER1913-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 61 kDApplication
Fig2: Sample: Kidney(Rat) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-ChRM1/Acetylcholine receptor(M1) (ER1913-39) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 51kD Observed band size : 51kD| Product Name | muscarinic Acetylcholine Receptor 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | muscarinic acetylcholine receptor M-AChR M1 Muscarinic Acetylcholine Receptor 1 cholinergic receptor, muscarinic 1 Chrm1 Chrm-1 M1 M1R Muscarinic Acetylcholine receptor M1 Cholinergic receptor muscarinic 1 CHRM 1 CHRM1 HM 1 HM1 M1 M1 muscarinic cholinergic receptor MGC30125 Muscarinic acetylcholine receptor M1 Acetylcholine receptor(M1). |
| Product description | The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. |
| Immunogen | KLH conjugated synthetic peptide derived from human muscarinic Acetylcholine Receptor 1 321-420/460 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | muscarinic acetylcholine receptor
M-AChR M1
Muscarinic Acetylcholine Receptor 1
cholinergic receptor muscarinic 1 Chrm1 Chrm-1 M1 M1R Muscarinic Acetylcholine receptor M1 Cholinergic receptor muscarinic 1 CHRM 1 CHRM1 HM 1 HM1 M1 M1 muscarinic cholinergic receptor MGC30125 Muscarinic acetylcholine receptor M1 Acetylcholine receptor(M1). |
|---|---|
| Molecular Weight(MW) | 51 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P11229 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-muscarinic Acetylcholine Receptor 1 (ER1913-39) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 61 kD
Application
Fig2: Sample: Kidney(Rat) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-ChRM1/Acetylcholine receptor(M1) (ER1913-39) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 51kD Observed band size : 51kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
