Application
Fig1: Tissue/cell: muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Fast skeletal Myosin Polyclonal Antibody, Unconjugated(ER1913-44) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MYLPF) Polyclonal Antibody, Unconjugated (ER1913-44) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Sample: A673(Human) Cell Lysate at 30 ug Primary: Anti-MYLPF (ER1913-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 19 kDApplication
Fig4: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-MYLPF (ER1913-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 19 kD| Product Name | MYLPF |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2410014J02Rik DKFZp779C0757 DTNB Fast skeletal myosin light chain 2 G2 HUMMLC2B MGC13450 MLC 2 MRLC2 MYL11 MLC2B MLC2F MLRS_HUMAN MYLPF Myosin light polypeptide 2 alkali Myosin regulatory light chain 2, skeletal muscle isoform. |
| Immunogen | KLH conjugated synthetic peptide derived from human Fast skeletal myosin light chain 2 101-170/170 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | 2410014J02Rik
DKFZp779C0757
DTNB
Fast skeletal myosin light chain 2
G2
HUMMLC2B
MGC13450
MLC 2
MRLC2
MYL11
MLC2B
MLC2F
MLRS_HUMAN
MYLPF
Myosin light polypeptide 2 alkali
Myosin regulatory light chain 2 skeletal muscle isoform. |
|---|---|
| Molecular Weight(MW) | 19 KDa |
| SwissProt ID | Q96A32 |
|---|
Application
Fig1: Tissue/cell: muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Fast skeletal Myosin Polyclonal Antibody, Unconjugated(ER1913-44) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MYLPF) Polyclonal Antibody, Unconjugated (ER1913-44) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Sample: A673(Human) Cell Lysate at 30 ug Primary: Anti-MYLPF (ER1913-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 19 kD
Application
Fig4: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-MYLPF (ER1913-44) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 19 kD| Positive Control | Expressed in fetal and adult skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
