Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Myosin light chain (phospho S20) (ER1913-46) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kDApplication
Fig2: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(ER1913-46) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(ER1913-46) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | Myosin light chain (phospho S20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MYL9 (phospho S20) p-MLC(Ser20) phospho-MLC(Ser20) p-Myosin light chain(Ser20) MYL9_HUMAN Myosin regulatory light polypeptide 9 20 kDa myosin light chain LC20 MLC-2C Myosin RLC Myosin regulatory light chain 2, smooth muscle isoform Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 MLC2 MRLC1 MYRL2. |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human MYL9 around the phosphorylation site of Ser20 AT(p-S)NV |
| Modification | p-S20 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | MYL9 (phospho S20)
p-MLC(Ser20)
phospho-MLC(Ser20)
p-Myosin light chain(Ser20)
MYL9_HUMAN
Myosin regulatory light polypeptide 9
20 kDa myosin light chain
LC20
MLC-2C
Myosin RLC
Myosin regulatory light chain 2 smooth muscle isoform Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 MLC2 MRLC1 MYRL2. |
|---|---|
| Molecular Weight(MW) | 20 KDa |
| SwissProt ID | P24844 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Myosin light chain (phospho S20) (ER1913-46) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD
Application
Fig2: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(ER1913-46) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(ER1913-46) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Smooth muscle tissues and in some, but not all, nonmuscle cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
