Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (ER1913-48) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (ER1913-48) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig3: Sample: Hela Cell Lysate at 40 ug Primary: Anti- NADPH oxidase 4 (ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kDApplication
Fig4: Sample: HepG2 Cell Lysate at 40 ug Primary: Anti- NADPH oxidase 4(ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kDApplication
Fig5: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- NADPH oxidase4 (ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kDApplication
Fig6: Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (ER1913-48) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kDApplication
Fig7: Blank control: Raji. Primary Antibody (green line): Rabbit Anti-NADPH oxidase 4 antibody (ER1913-48) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig8: Sample: muscle (Mouse) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kDApplication
Fig9: Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD| Product Name | NADPH oxidase 4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KOX 1 KOX Nox 4 Nox-4 NADPH oxidase 4 RENOX Kidney oxidase-1 Kidney superoxide-producing NADPH oxidase Kox-1 NADPH Nox4 NOX4_HUMAN Renal NAD(P)H-oxidase RENOX. |
| Product description | Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nox-4 81-180/578 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | KOX 1 KOX Nox 4 Nox-4 NADPH oxidase 4 RENOX Kidney oxidase-1 Kidney superoxide-producing NADPH oxidase Kox-1 NADPH Nox4 NOX4_HUMAN Renal NAD(P)H-oxidase RENOX. |
|---|---|
| Molecular Weight(MW) | 64 KDa |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Cell membrane. Note=May localize to plasma membrane and focal adhesions. |
| SwissProt ID | Q9JHI8 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (ER1913-48) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (ER1913-48) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig3: Sample: Hela Cell Lysate at 40 ug Primary: Anti- NADPH oxidase 4 (ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Application
Fig4: Sample: HepG2 Cell Lysate at 40 ug Primary: Anti- NADPH oxidase 4(ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Application
Fig5: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- NADPH oxidase4 (ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Application
Fig6: Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (ER1913-48) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Application
Fig7: Blank control: Raji. Primary Antibody (green line): Rabbit Anti-NADPH oxidase 4 antibody (ER1913-48) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig8: Sample: muscle (Mouse) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD
Application
Fig9: Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (ER1913-48) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD| Positive Control | EXpressed in brain, in all layers of the cerebellum, in pyramidal cells of the Ammon horn and in Purkinje cells (at protein level). Expressed in osteoclasts, leukocytes, kidney, liver and lung. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
