Application
Fig1: Protein: heart(mouse) lysate at 40ug; Primary: rabbit Anti-Nanos3 (ER1913-51) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 19 kD Observed band size: 17 kD| Product Name | Nanos3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NANOS3 NANOS 3 NANOS-3 NOS3 NOS-3 Nanos homolog 3 NANO3_HUMAN. |
| Product description | Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nanos3 101-173/173 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | NANOS3 NANOS 3 NANOS-3 NOS3 NOS-3 Nanos homolog 3 NANO3_HUMAN. |
|---|---|
| Molecular Weight(MW) | 19 KDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. |
| SwissProt ID | P60323 |
|---|
Application
Fig1: Protein: heart(mouse) lysate at 40ug; Primary: rabbit Anti-Nanos3 (ER1913-51) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 19 kD Observed band size: 17 kD| Positive Control | Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
