Application
Fig1: Sample: SP2/0 (Mouse) Lysate at 30 ug Primary: Anti-NARG1 (ER1913-54) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 101 kD Observed band size: 101 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NARG1) Polyclonal Antibody, Unconjugated (ER1913-54) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NARG1) Polyclonal Antibody, Unconjugated (ER1913-54) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.| Product Name | NARG1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5730450D16Rik 6330400I15 ASTBDN GA19 Gastric cancer antigen Ga19 MGC29428 mNAT1 N alpha acetyltransferase 15, NatA auxiliary subunit N terminal acetyltransferase N(alpha) acetyltransferase 15, NatA auxiliary subunit N-alpha-acetyltransferase 15 N-terminal acetyltransferase Naa15 NAA15_HUMAN NatA auxiliary subunit NATH NMDA receptor regulated 1 NMDA receptor regulated protein 1 NMDA receptor-regulated protein 1 Protein tubedown 1 Protein tubedown-1 Tbdn 1 TBDN100 Transcriptional coactivator tubedown 100 tubedown-1. |
| Product description | Auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter. |
| Immunogen | KLH conjugated synthetic peptide derived from human NARG1 121-220/866 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitZebra FishSheep |
| Concentration | 1mg/ml |
| Alternative Names | 5730450D16Rik
6330400I15
ASTBDN
GA19
Gastric cancer antigen Ga19
MGC29428
mNAT1
N alpha acetyltransferase 15 NatA auxiliary subunit N terminal acetyltransferase N(alpha) acetyltransferase 15 NatA auxiliary subunit N-alpha-acetyltransferase 15 N-terminal acetyltransferase Naa15 NAA15_HUMAN NatA auxiliary subunit NATH NMDA receptor regulated 1 NMDA receptor regulated protein 1 NMDA receptor-regulated protein 1 Protein tubedown 1 Protein tubedown-1 Tbdn 1 TBDN100 Transcriptional coactivator tubedown 100 tubedown-1. |
|---|---|
| Molecular Weight(MW) | 101 KDa |
| Cellular Localization | Cytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes. |
| SwissProt ID | Q9BXJ9 |
|---|
Application
Fig1: Sample: SP2/0 (Mouse) Lysate at 30 ug Primary: Anti-NARG1 (ER1913-54) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 101 kD Observed band size: 101 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NARG1) Polyclonal Antibody, Unconjugated (ER1913-54) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NARG1) Polyclonal Antibody, Unconjugated (ER1913-54) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.| Positive Control | Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
