Application
Fig1: Sample: Small intestine (Mouse) Lysate at 40 ug Primary: Anti- NDUFS2 (ER1913-59) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kDApplication
Fig2: Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-NDUFS2 (ER1913-59) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD| Product Name | NDUFS2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDUS2_HUMAN NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Complex I-49kD CI-49kD NADH-ubiquinone oxidoreductase 49 kDa subunit NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial isoform 1 precursor CI-49. |
| Product description | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Immunogen | KLH conjugated synthetic peptide derived from human NDUFS2 351-450/463 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | NDUS2_HUMAN
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 mitochondrial Complex I-49kD CI-49kD NADH-ubiquinone oxidoreductase 49 kDa subunit NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 mitochondrial isoform 1 precursor CI-49. |
|---|---|
| Molecular Weight(MW) | 49 KDa |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
| SwissProt ID | O75306 |
|---|
Application
Fig1: Sample: Small intestine (Mouse) Lysate at 40 ug Primary: Anti- NDUFS2 (ER1913-59) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD
Application
Fig2: Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-NDUFS2 (ER1913-59) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
