Application
Fig1: Sample: HUVEC(Human) Cell Lysate at 30 ug Primary: Anti- Nectin 3 (ER1913-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig2: Sample: Epididymis (Mouse) Lysate at 40 ug Primary: Anti- Nectin 3 (ER1913-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Product Name | Nectin 3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD113 CDw113 DKFZP566B0846 FLJ90624 Nectin-3 Nectin3 Poliovirus receptor related 3 Poliovirus receptor related protein 3 Poliovirus receptor-related protein 3 PPR3 PRR3 PVRL 3 PVRL3 PVRL3_HUMAN PVRR3. |
| Product description | Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nectin 3 431-549/549 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CD113 CDw113 DKFZP566B0846 FLJ90624 Nectin-3 Nectin3 Poliovirus receptor related 3 Poliovirus receptor related protein 3 Poliovirus receptor-related protein 3 PPR3 PRR3 PVRL 3 PVRL3 PVRL3_HUMAN PVRR3. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Cell membrane; Single-pass membrane protein (Potential). |
| SwissProt ID | Q9NQS3 |
|---|
Application
Fig1: Sample: HUVEC(Human) Cell Lysate at 30 ug Primary: Anti- Nectin 3 (ER1913-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig2: Sample: Epididymis (Mouse) Lysate at 40 ug Primary: Anti- Nectin 3 (ER1913-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Positive Control | Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
