Application
Fig1: Sample: Skin (Mouse) Lysate at 40 ug Primary: Anti-Nectin 4 (ER1913-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kDApplication
Fig2: Sample: A431(Human) Cell Lysate at 30 ug Thymus (Mouse) Lysate at 40 ug Primary: Anti- Nectin 4 (ER1913-61) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 55 kDApplication
Fig3: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti- Nectin 4 (ER1913-61) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 55 kDApplication
Fig4: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-Nectin 4 (bs- 10029R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD| Product Name | Nectin 4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ig superfamily receptor LNIR Nectin 4 poliovirus receptor-related 4 PRR4 poliovirus receptor related 4 DKFZp686K05193 EDSS1 Nectin-4 poliovirus receptor-related 4 Processed poliovirus receptor-related protein 4 pvrl4 PVRL4_HUMAN. |
| Product description | Self-associates. Interacts via its Ig-like V-type domain with PVRL1/nectin-1 Ig-like V-type domain. Interacts via its C-terminus with MLLT4. Binds to Measles virus protein H and acts as a receptor for this virus. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nectin 4 151-250/510 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Ig superfamily receptor LNIR Nectin 4 poliovirus receptor-related 4 PRR4 poliovirus receptor related 4 DKFZp686K05193 EDSS1 Nectin-4 poliovirus receptor-related 4 Processed poliovirus receptor-related protein 4 pvrl4 PVRL4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 53 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (Potential). Cell junction, adherens junction. Processed poliovirus receptor-related protein 4: Secreted. |
| SwissProt ID | Q96NY8 |
|---|
Application
Fig1: Sample: Skin (Mouse) Lysate at 40 ug Primary: Anti-Nectin 4 (ER1913-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD
Application
Fig2: Sample: A431(Human) Cell Lysate at 30 ug Thymus (Mouse) Lysate at 40 ug Primary: Anti- Nectin 4 (ER1913-61) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 55 kD
Application
Fig3: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti- Nectin 4 (ER1913-61) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 55 kD
Application
Fig4: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-Nectin 4 (bs- 10029R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD| Positive Control | Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
