Application
Fig1: Sample: Hela (Human)Cell Lysate at 40 ug Primary: Anti- NEK11(ER1913-62)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 54'74kD Observed band size: 70kD| Product Name | NEK11 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 4932416N14Rik FLJ23495 NEK11 NEK11_HUMAN NEK11S/L Never in mitosis A-related kinase 11 NIMA (never in mitosis gene a) related kinase 11 NIMA (never in mitosis gene a)- related kinase 11 NIMA related kinase 11L NIMA related kinase 11S NIMA-related kinase 11 NimA-related protein kinase 11 Serine/threonine-protein kinase Nek11. |
| Product description | Possible role in S-phase checkpoint mechanism. |
| Immunogen | KLH conjugated synthetic peptide derived from human NEK11 151-250/645 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | 4932416N14Rik FLJ23495 NEK11 NEK11_HUMAN NEK11S/L Never in mitosis A-related kinase 11 NIMA (never in mitosis gene a) related kinase 11 NIMA (never in mitosis gene a)- related kinase 11 NIMA related kinase 11L NIMA related kinase 11S NIMA-related kinase 11 NimA-related protein kinase 11 Serine/threonine-protein kinase Nek11. |
|---|---|
| Molecular Weight(MW) | 54 and 74 KDa |
| Cellular Localization | Nucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells. |
| SwissProt ID | Q8NG66 |
|---|
Application
Fig1: Sample: Hela (Human)Cell Lysate at 40 ug Primary: Anti- NEK11(ER1913-62)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 54'74kD Observed band size: 70kD| Positive Control | Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
