Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (ER1913-64) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (ER1913-64) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: Hepg2(Human) Cell Lysate at 30 ug Primary: Anti-Nesprin 3 (ER1913-64) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 112 kD Observed band size: 112 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (ER1913-64) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Nesprin 3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C14orf49 Chromosome 14 open reading frame 49 FLJ25605 MGC75267 Nesp3 Nesprin3 Nesprin-3 NET53 Nuclear Envelope Spectrin Repeat Protein 3 Spectrin Repeat Containing, Nuclear Envelope Family Member 3 SYNE3 SYNE3_HUMAN. |
| Product description | Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nesprin 3 201-300/975 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | C14orf49
Chromosome 14 open reading frame 49
FLJ25605
MGC75267
Nesp3
Nesprin3
Nesprin-3
NET53
Nuclear Envelope Spectrin Repeat Protein 3
Spectrin Repeat Containing Nuclear Envelope Family Member 3 SYNE3 SYNE3_HUMAN. |
|---|---|
| Molecular Weight(MW) | 112 KDa |
| Cellular Localization | Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells. |
| SwissProt ID | Q6ZMZ3 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (ER1913-64) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (ER1913-64) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: Hepg2(Human) Cell Lysate at 30 ug Primary: Anti-Nesprin 3 (ER1913-64) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 112 kD Observed band size: 112 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (ER1913-64) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
