Application
Fig1: Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Neurogenin 3 Polyclonal Antibody, Unconjugated(ER1913-71) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample:Liver(Mouse) Lysate at 30 ug Primary: Anti-Neurogenin3 (ER1913-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 26 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Neurogenin 3) Polyclonal Antibody, Unconjugated (ER1913-71) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Neurogenin 3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NGN3 neurogenin 3 Neurog3 Neurogenin3 Neurogenin-3 ATH4B Atoh5 Math4B NEUROG3 ngn3 OTTHUMP00000019730 Protein atonal homolog 5. |
| Product description | Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. |
| Immunogen | KLH conjugated synthetic peptide derived from human NGN3 61-160/214 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | NGN3 neurogenin 3 Neurog3 Neurogenin3 Neurogenin-3 ATH4B Atoh5 Math4B NEUROG3 ngn3 OTTHUMP00000019730 Protein atonal homolog 5. |
|---|---|
| Molecular Weight(MW) | 23 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9Y4Z2 |
|---|
Application
Fig1: Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Neurogenin 3 Polyclonal Antibody, Unconjugated(ER1913-71) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample:Liver(Mouse) Lysate at 30 ug Primary: Anti-Neurogenin3 (ER1913-71) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 26 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Neurogenin 3) Polyclonal Antibody, Unconjugated (ER1913-71) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
