Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Neuroligin 1 (ER1913-74) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 87 kDApplication
Fig2: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Neuroligin 1 (ER1913-74) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 87 kDApplication
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-Neuroligin 1 (ER1913-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 87 kDApplication
Fig4: Blank control(blue): Mouse brain cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti- Neuroligin 1 /PE Conjugated antibody (ER1913-74 /PE), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/PE(orange) ,used under the same conditions.Application
Fig5: Blank control (blue line): U251 (fixed with 70% ethanol for 5 min at -20℃). Primary Antibody (green line): Rabbit Anti- Neuroligin 1 antibody (ER1913-74), Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.| Product Name | Neuroligin 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NLG 1 KIAA1070 MGC45115 NLG1 Neuroligin-1 NLGN1 NLGN1_HUMAN. |
| Product description | Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. |
| Immunogen | KLH conjugated synthetic peptide derived from human Neuroligin 1 701-800/863 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | NLG 1 KIAA1070 MGC45115 NLG1 Neuroligin-1 NLGN1 NLGN1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 87 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. Detected at dendritic spines. Colocalized with DLG4/PSD-95 and GRIN1/NMDAR1. |
| SwissProt ID | Q8N2Q7 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Neuroligin 1 (ER1913-74) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 87 kD
Application
Fig2: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Neuroligin 1 (ER1913-74) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 87 kD
Application
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-Neuroligin 1 (ER1913-74) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 87 kD
Application
Fig4: Blank control(blue): Mouse brain cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti- Neuroligin 1 /PE Conjugated antibody (ER1913-74 /PE), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/PE(orange) ,used under the same conditions.
Application
Fig5: Blank control (blue line): U251 (fixed with 70% ethanol for 5 min at -20℃). Primary Antibody (green line): Rabbit Anti- Neuroligin 1 antibody (ER1913-74), Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.| Positive Control | Expressed in the blood vessel walls (at protein level). Detected in brain, and at lower levels in pancreas islet beta cells. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
