Application
Fig1: Sample: Kidney(Mouse) lysate at 30ug; Brain(Mouse) lysate at 30ug Primary: Anti-NFkB Inducing Kinase NIK (ER1913-80) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 104kD Observed band size : 104kDApplication
Fig2: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSNIK Polyclonal Antibody, Unconjugated(ER1913-80) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Blank control: A431. Primary Antibody (green line): Rabbit Anti-NFkB Inducing Kinase NIK antibody (ER1913-80) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | NFkB Inducing Kinase NIK |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NF-kappaB-Inducing Kinase NFkB Inducing Kinase NIK NF kappa beta inducing kinase NF-kappa-beta-inducing kinase HS HSNIK NIK Serine/threonine-protein kinase NIK mitogen-activated protein kinase kinase kinase 14 MAPKKK14 MAP3K14 FTDCR1B NF-kappa-beta-inducing kinase M3K14_HUMAN. |
| Product description | Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. |
| Immunogen | KLH conjugated synthetic peptide derived from human NIK 901-960/947 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | NF-kappaB-Inducing Kinase NFkB Inducing Kinase NIK NF kappa beta inducing kinase NF-kappa-beta-inducing kinase HS HSNIK NIK Serine/threonine-protein kinase NIK mitogen-activated protein kinase kinase kinase 14 MAPKKK14 MAP3K14 FTDCR1B NF-kappa-beta-inducing kinase M3K14_HUMAN. |
|---|---|
| Molecular Weight(MW) | 104 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q99558 |
|---|
Application
Fig1: Sample: Kidney(Mouse) lysate at 30ug; Brain(Mouse) lysate at 30ug Primary: Anti-NFkB Inducing Kinase NIK (ER1913-80) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 104kD Observed band size : 104kD
Application
Fig2: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSNIK Polyclonal Antibody, Unconjugated(ER1913-80) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Blank control: A431. Primary Antibody (green line): Rabbit Anti-NFkB Inducing Kinase NIK antibody (ER1913-80) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
