Application
Fig1: Blank control (blue line): Mouse thymus cells (blue). Primary Antibody (green line): Rabbit Anti-NKG2D antibody (ER1913-86) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- NKG2D (ER1913-86) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 26 kD| Product Name | NKG2D |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KLRK1 natural killer cell group 2D NKG2-D isoform b Klrk1 Nkrp2 NKG2-D type II integral membrane protein NKG2-D-activating NK receptor NK cell receptor D Killer cell lectin-like receptor subfamily K NK lectin-like receptor CD314 CD 314 CD314 antigen D12S2489E Killer cell lectin like receptor subfamily K member 1 Killer cell lectin-like receptor subfamily K member 1 KLR Klrk1 NKG2 D activating NK receptor NKG2 D type II integral membrane protein NKG2-D-activating NK receptor NKG2D_HUMAN NKLLR NKR P2 Nkrp2. |
| Product description | Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. |
| Immunogen | KLH conjugated synthetic peptide derived from human NKG2D 121-219/219 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | KLRK1 natural killer cell group 2D NKG2-D isoform b Klrk1 Nkrp2 NKG2-D type II integral membrane protein NKG2-D-activating NK receptor NK cell receptor D Killer cell lectin-like receptor subfamily K NK lectin-like receptor CD314 CD 314 CD314 antigen D12S2489E Killer cell lectin like receptor subfamily K member 1 Killer cell lectin-like receptor subfamily K member 1 KLR Klrk1 NKG2 D activating NK receptor NKG2 D type II integral membrane protein NKG2-D-activating NK receptor NKG2D_HUMAN NKLLR NKR P2 Nkrp2. |
|---|---|
| Molecular Weight(MW) | 25 KDa |
| Cellular Localization | Membrane; Single-pass type II membrane protein. |
| SwissProt ID | O70215 |
|---|
Application
Fig1: Blank control (blue line): Mouse thymus cells (blue). Primary Antibody (green line): Rabbit Anti-NKG2D antibody (ER1913-86) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- NKG2D (ER1913-86) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 26 kD| Positive Control | Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs). |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
