Application
Fig1: Sample: A549 Cell Lysate at 40 ug Primary: Anti- NMDAR2B (ER1913-90) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 163 kD Observed band size: 210 kDApplication
Fig2: Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(ER1913-90) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(ER1913-90) 1:300, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | NMDAR2B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NMDA2B (epsilon 2) GRIN 2B GRIN2B hNR 3 hNR3 MGC142178 MGC142180 N methyl D asparate receptor channel subunit epsilon 2 N METHYL D ASPARTATE RECEPTOR CHANNEL SUBUNIT EPSILON 2 N methyl D aspartate receptor subtype 2B N methyl D aspartate receptor subunit 2B N methyl D aspartate receptor subunit 3 NMDA NR2B NMDA R2B Nmdar2b NMDE2 NME2 NR2B NR3 N-Methyl-d-Asprtate receptor 2B AW490526 Glutamate [NMDA] receptor subunit epsilon 2 Glutamate Receptor Ionotropic N Methyl D Aspartate 2B Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B Glutamate receptor ionotropic NMDA2B Glutamate receptor subunit epsilon 2 Glutamate receptor, ionotropic NMDE2_HUMAN glutamate receptor ionotropic, NMDA 2B precursor. |
| Product description | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. |
| Immunogen | KLH conjugated synthetic peptide derived from human NMDAR2B 451-550/1484 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: ICC:1:100-500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | NMDA2B (epsilon 2)
GRIN 2B
GRIN2B
hNR 3
hNR3
MGC142178
MGC142180
N methyl D asparate receptor channel subunit epsilon 2
N METHYL D ASPARTATE RECEPTOR CHANNEL SUBUNIT EPSILON 2
N methyl D aspartate receptor subtype 2B
N methyl D aspartate receptor subunit 2B
N methyl D aspartate receptor subunit 3
NMDA NR2B
NMDA R2B
Nmdar2b
NMDE2
NME2
NR2B
NR3
N-Methyl-d-Asprtate receptor 2B
AW490526
Glutamate [NMDA] receptor subunit epsilon 2
Glutamate Receptor Ionotropic N Methyl D Aspartate 2B
Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B
Glutamate receptor ionotropic NMDA2B
Glutamate receptor subunit epsilon 2
Glutamate receptor ionotropic NMDE2_HUMAN glutamate receptor ionotropic NMDA 2B precursor. |
|---|---|
| Molecular Weight(MW) | 163 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q13224 |
|---|
Application
Fig1: Sample: A549 Cell Lysate at 40 ug Primary: Anti- NMDAR2B (ER1913-90) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 163 kD Observed band size: 210 kD
Application
Fig2: Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(ER1913-90) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated(ER1913-90) 1:300, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: ICC:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
