Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Nmnat1 (ER1913-92) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kDApplication
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-Nmnat1 (ER1913-92) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD| Product Name | Nmnat1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NaMN adenylyltransferase 1 Nicotinamide mononucleotide adenylyltransferase 1 nicotinamide nucleotide adenylyltransferase 1 nicotinate nucleotide adenylyltransferase 1 Nicotinate-nucleotide adenylyltransferase 1 NMN adenylyltransferase 1 NMNA1_HUMAN Nmnat 1 Nmnat1 Nmnat-1 OTTHUMP00000001731 OTTHUMP00000001732 OTTHUMP00000035892 PNAT 1 PNAT1 pyridine nucleotide adenylyltransferase 1. |
| Product description | Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following mechanical or toxic insults. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nmnat1 201-279/279 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | NaMN adenylyltransferase 1 Nicotinamide mononucleotide adenylyltransferase 1 nicotinamide nucleotide adenylyltransferase 1 nicotinate nucleotide adenylyltransferase 1 Nicotinate-nucleotide adenylyltransferase 1 NMN adenylyltransferase 1 NMNA1_HUMAN Nmnat 1 Nmnat1 Nmnat-1 OTTHUMP00000001731 OTTHUMP00000001732 OTTHUMP00000035892 PNAT 1 PNAT1 pyridine nucleotide adenylyltransferase 1. |
|---|---|
| Molecular Weight(MW) | 32 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9HAN9 |
|---|
Application
Fig1: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Nmnat1 (ER1913-92) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-Nmnat1 (ER1913-92) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD| Positive Control | Widely expressed with highest levels in skeletal muscle, heart and kidney. Also expressed in the liver pancreas and placenta. Widely expressed throughout the brain. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
