Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Nociceptin receptor (ER1913-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 52 kDApplication
Fig2: Sample: Heart(Rat) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-Nociceptin receptor (ER1913-94) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 40kD Observed band size : 40kDApplication
Fig3: Immunofluorescence. Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(ER1913-94) 1:200, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37℃.| Product Name | Nociceptin receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H.sapiens mRNA for ORL1 receptor Kappa type 3 opioid receptor Kappa-type 3 opioid receptor KOR 3 KOR-3 KOR3 LC132 receptor like MGC34578 Nociceptin receptor NOCIR OOR Opiate receptor like 1 Opiod receptor like 1 Oprl1 OPRX_HUMAN ORL1 Orphanin FQ receptor. |
| Product description | Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nociceptin receptor 1-47/370 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | H.sapiens mRNA for ORL1 receptor Kappa type 3 opioid receptor Kappa-type 3 opioid receptor KOR 3 KOR-3 KOR3 LC132 receptor like MGC34578 Nociceptin receptor NOCIR OOR Opiate receptor like 1 Opiod receptor like 1 Oprl1 OPRX_HUMAN ORL1 Orphanin FQ receptor. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P35377 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Nociceptin receptor (ER1913-94) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 52 kD
Application
Fig2: Sample: Heart(Rat) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-Nociceptin receptor (ER1913-94) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 40kD Observed band size : 40kD
Application
Fig3: Immunofluorescence. Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(ER1913-94) 1:200, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37℃.| Positive Control | In the brain, isoform KOR3 and isoform KOR3C are most abundant in hypothalamus and periaqueductal gray. Isoform KOR3A is highly expressed in cortex, striatum and brainstem. Isoform KOR3D is highly expressed in cerebellum, hypothalamus and brainstem. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
