Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Nox1 (ER1913-99) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kDApplication
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- Nox1 (ER1913-99) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 62 kD Observed band size: 57 kDApplication
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Uterus (Mouse) Lysate at 40 ug DU145(Human) Cell Lysate at 30 ug Primary: Anti- Nox1 (ER1913-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 60 kD| Product Name | Nox1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogenic oxidase 1 MOX1 NADH/NADPH mitogenic oxidase subunit P65 MOX NADPH oxidase 1 NADPH oxidase 1 variant NOH 1L NADPH oxidase homolog 1 NOH 1 NOH1 RP1-146H21.1 GP91-2 Mitogenic oxidase (pyridine nucleotide dependent superoxide generating). |
| Immunogen | KLH conjugated synthetic peptide derived from human Nox1 425-515/515 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Mitogenic oxidase 1 MOX1 NADH/NADPH mitogenic oxidase subunit P65 MOX NADPH oxidase 1 NADPH oxidase 1 variant NOH 1L NADPH oxidase homolog 1 NOH 1 NOH1 RP1-146H21.1 GP91-2 Mitogenic oxidase (pyridine nucleotide dependent superoxide generating). |
|---|---|
| Molecular Weight(MW) | 62 KDa |
| Cellular Localization | Cell projection, invadopodium membrane; Multi-pass membrane protein. |
| SwissProt ID | Q9Y5S8 |
|---|
Application
Fig1: Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Nox1 (ER1913-99) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- Nox1 (ER1913-99) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 62 kD Observed band size: 57 kD
Application
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Uterus (Mouse) Lysate at 40 ug DU145(Human) Cell Lysate at 30 ug Primary: Anti- Nox1 (ER1913-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 60 kD| Positive Control | NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
