Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-NOX5 (ER1914-01) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 64 kD| Product Name | NOX5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NADPH oxidase 5 NADPH oxidase EFhand calcium binding domain 5 NOX5 NOX5_HUMAN MGC149776 MGC149777. |
| Product description | Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. |
| Immunogen | KLH conjugated synthetic peptide derived from human NOX5 675-765/765 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | NADPH oxidase 5 NADPH oxidase EFhand calcium binding domain 5 NOX5 NOX5_HUMAN MGC149776 MGC149777. |
|---|---|
| Molecular Weight(MW) | 84 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein (Potential). Isoform v2: Endoplasmic reticulum. Isoform v5: Endoplasmic reticulum. |
| SwissProt ID | Q96PH1 |
|---|
Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-NOX5 (ER1914-01) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 64 kD| Positive Control | Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
