Aminopeptidases are widely distributed in eukaryotes and prokaryotes. These enzymes catalyze the removal of amino acids from the N-termini of proteins or peptide substrates. Aminopeptidases are involved in regulating hormone levels and are essential for digestive and intracellular protein metabolism. NPEPL1, also referred to as aminopeptidase-like 1, is a 523 amino acid protein that belongs to the peptidase M17 family and is ubiquitously expressed. NPEPL1 may be involved in the processing, catabolism and degradation of intracellular proteins by catalyzing the removal of unsubstituted N-terminal amino acids from various peptides. NPEPL1 contains several zinc binding sites and and is expressed as three isoforms due to alternative splicing events.
Immunogen
KLH conjugated synthetic peptide derived from human NPEPL1 371-470/494
Fig1: Sample: lung (mouse) Lysate at 40 ug
Primary: Anti-NPEPL1(ER1914-05) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Positive Control
Ubiquitously expressed.
Application Notes
WB:1:500-2000:
Additional Information
Form
Liquid
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.