Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (ER1914-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-NPFF1 Receptor (ER1914-06) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (ER1914-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | NPFF1 Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPFF1 Receptor G Protein Coupled Receptor 147 G-protein coupled receptor 147 GPR147 Neuropeptide FF receptor 1 NPFF1 NPFF1_HUMAN NPFF1R1 NPFFR1 RFamide related peptide receptor OT7T022 RFamide-related peptide receptor OT7T022. |
| Product description | The neuropeptide FF receptors are members of the G-protein coupled receptor superfamily of integral membrane proteins which bind the pain modulatory neuropeptides AF and FF. The Neuropeptide FF receptor family is a member of the G protein-coupled receptor superfamily containing two subtypes, NPFF1 and NPFF2, which exhibit a high affinity for Neuropeptide FF (NPFF) peptides. NPFF1 is broadly distributed in the central nervous system with the highest levels found in the limbic system and the hypothalamus. NPFF2 is present in high density, particularly in mammals in the superficial layers of the spinal cord where it is involved in nociception and modulation of opioid functions These receptors participate to the modulation of opioid receptor function in the brain and spinal cord, and can either reduce or increase opioid receptor function depending which tissue they are released in, reflecting a complex role for neuropeptide FF in pain responses. |
| Immunogen | KLH conjugated synthetic peptide derived from human GPR147/NPFF1 Receptor 151-260/430 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | NPFF1 Receptor
G Protein Coupled Receptor 147
G-protein coupled receptor 147 GPR147 Neuropeptide FF receptor 1 NPFF1 NPFF1_HUMAN NPFF1R1 NPFFR1 RFamide related peptide receptor OT7T022 RFamide-related peptide receptor OT7T022. |
|---|---|
| Molecular Weight(MW) | 48 KDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q9GZQ6 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (ER1914-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-NPFF1 Receptor (ER1914-06) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (ER1914-06) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Rat brain tissue, U251(Human) Cell Lysate, Mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
