Application
Fig1: Sample: HL-60 (Human) Cell Lysate at 40 ug A431 (Human) Cell Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Primary: Anti-NPRC (ER1914-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NPRC) Polyclonal Antibody, Unconjugated (ER1914-07) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | NPRC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANP C ANPC ANPRC Atrial natriuretic peptide C type receptor Atrionatriuretic peptide receptor C Natriuretic peptide receptor C/guanylate cyclase C NPR 3 NPR C NPR3 NPRC ANPRC_HUMAN. |
| Product description | Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human NPR-C 301-400/541 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | ANP C ANPC ANPRC Atrial natriuretic peptide C type receptor Atrionatriuretic peptide receptor C Natriuretic peptide receptor C/guanylate cyclase C NPR 3 NPR C NPR3 NPRC ANPRC_HUMAN. |
|---|---|
| Molecular Weight(MW) | 57 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | P17342 |
|---|
Application
Fig1: Sample: HL-60 (Human) Cell Lysate at 40 ug A431 (Human) Cell Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Primary: Anti-NPRC (ER1914-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NPRC) Polyclonal Antibody, Unconjugated (ER1914-07) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
