Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Hela KO NRSF (Human) Cell Lysate at 30 ug Primary: Anti- NRSF (ER1914-14) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kDApplication
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Rest c(Human) Cell Lysate at 30 ug Primary: Anti-NRSF (ER1914-14) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kDApplication
Fig3: Sample: Raji Cell Lysate at 40 ug Primary: Anti- NRSF (ER1914-14) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kDApplication
Fig4: Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-NRSF (ER1914-14) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD| Product Name | NRSF |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RE1-Silencing Transcription Factor NRSF Neural-Restrictive Silencer Factor XBR Neuron Restrictive Silencer Factor Repressor Binding To The X2 Box X2 Box Repressor REST_HUMAN |
| Product description | Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. |
| Immunogen | KLH conjugated synthetic peptide derived from human NRSF 531-450/1097 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | RE1-Silencing Transcription Factor NRSF Neural-Restrictive Silencer Factor XBR Neuron Restrictive Silencer Factor Repressor Binding To The X2 Box X2 Box Repressor REST_HUMAN |
|---|---|
| Molecular Weight(MW) | 121 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q13127 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Hela KO NRSF (Human) Cell Lysate at 30 ug Primary: Anti- NRSF (ER1914-14) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD
Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Rest c(Human) Cell Lysate at 30 ug Primary: Anti-NRSF (ER1914-14) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD
Application
Fig3: Sample: Raji Cell Lysate at 40 ug Primary: Anti- NRSF (ER1914-14) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD
Application
Fig4: Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-NRSF (ER1914-14) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 121 kD Observed band size: 121 kD| Positive Control | Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
