Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- Nuclear Pore Complex Proteins'Nup107 (ER1914-18)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 106kD Observed band size: 106kDApplication
Fig2: Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Nuclear Pore Complex Proteins/Nup107/AF488 Conjugated antibody (ER1914-18-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.| Product Name | Nuclear Pore Complex Proteins/Nup107 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 107 kDa nucleoporin NPC proteins NU107_HUMAN Nuclear pore complex protein Nup107 Nucleoporin 107kDa Nucleoporin Nup107 NUP 107 NUP 84 NUP107 NUP153 NUP155 NUP84 NUP98. |
| Product description | Essential component of nuclear pore complex. Required for the assembly of peripheral proteins into the nuclear pore complex. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nuclear Pore Complex Proteins 501-600/925 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3μg/Test: |
|
| Species Reactivity | HumanMouseRatPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | 107 kDa nucleoporin NPC proteins NU107_HUMAN Nuclear pore complex protein Nup107 Nucleoporin 107kDa Nucleoporin Nup107 NUP 107 NUP 84 NUP107 NUP153 NUP155 NUP84 NUP98. |
|---|---|
| Molecular Weight(MW) | 106 KDa |
| Cellular Localization | Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Note=Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores. |
| SwissProt ID | P57740 |
|---|
Application
Fig1: Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- Nuclear Pore Complex Proteins'Nup107 (ER1914-18)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 106kD Observed band size: 106kD
Application
Fig2: Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Nuclear Pore Complex Proteins/Nup107/AF488 Conjugated antibody (ER1914-18-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.| Application Notes | WB:1:500-2000: FC:3μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
