Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Nucleophosmin (ER1914-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kDApplication
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Nucleophosmin (ER1914-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kDApplication
Fig3: Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti-Nucleophosmin (ER1914-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kDApplication
Fig4: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NPM1 Polyclonal Antibody, Unconjugated(ER1914-19) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | Nucleophosmin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B23 B23 MGC104254 NMP1 NMP1 NO38 NPM 1 NPM NPM_HUMAN NPM1 Nucleolar Phosphoprotein B23 Nucleolar Phosphoprotein B23 Nucleolar protein NO38 Nucleophosmin (nucleolar phosphoprotein B23 numatrin) Nucleophosmin Nucleophosmin/B23.2 Nucleophosmin/nucleoplasmin family member 1 Nucleoplasmin Family Member 1 Nucleoplasmin Family Member 1 Numatrin Numatrin OTTHUMP00000161024 OTTHUMP00000161025 OTTHUMP00000223397 OTTHUMP00000223398 TRK fused gene. |
| Product description | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nucleophosmin 21-120/259 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | B23
B23
MGC104254 NMP1 NMP1 NO38 NPM 1 NPM NPM_HUMAN NPM1 Nucleolar Phosphoprotein B23 Nucleolar Phosphoprotein B23 Nucleolar protein NO38 Nucleophosmin (nucleolar phosphoprotein B23 numatrin) Nucleophosmin Nucleophosmin/B23.2 Nucleophosmin/nucleoplasmin family member 1 Nucleoplasmin Family Member 1 Nucleoplasmin Family Member 1 Numatrin Numatrin OTTHUMP00000161024 OTTHUMP00000161025 OTTHUMP00000223397 OTTHUMP00000223398 TRK fused gene. |
|---|---|
| Molecular Weight(MW) | 33 KDa |
| Cellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co-localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. |
| SwissProt ID | P06748 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Nucleophosmin (ER1914-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Nucleophosmin (ER1914-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Application
Fig3: Sample: Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti-Nucleophosmin (ER1914-19) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
Application
Fig4: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NPM1 Polyclonal Antibody, Unconjugated(ER1914-19) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Hela(Human) Cell Lysate, HepG2(Human) Cell Lysate, Raw264.7(Mouse) Cell Lysate, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
