Application
Fig1: Blank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Nucleoporin p62 antibody (ER1914-20) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (Nucleoporin p62) Polyclonal/MonoclonalAntibody, Unconjugated (ER1914-20) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Product Name | Nucleoporin p62 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NUP62_HUMAN 62 kDa nucleoporin DKFZp547L134 FLJ20822 FLJ43869 MGC841 Nuclear pore glycoprotein p62 nucleoporin 62kDa nucleoporin p62 nucleoporin p62KD NUP62 NUP62 protein Nucleoporin Nup62 p62. |
| Product description | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex. Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation. It might be involved in protein recruitment to the centrosome after nuclear breakdown. |
| Immunogen | KLH conjugated synthetic peptide derived from human Nucleoporin p62 451-522/522 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | NUP62_HUMAN
62 kDa nucleoporin
DKFZp547L134 FLJ20822 FLJ43869 MGC841 Nuclear pore glycoprotein p62 nucleoporin 62kDa nucleoporin p62 nucleoporin p62KD NUP62 NUP62 protein Nucleoporin Nup62 p62. |
|---|---|
| Molecular Weight(MW) | 53 KDa |
| Cellular Localization | Nucleus, nuclear pore complex. Cytoplasm, cytoskeleton, spindle pole. |
| SwissProt ID | P37198 |
|---|
Application
Fig1: Blank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Nucleoporin p62 antibody (ER1914-20) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (Nucleoporin p62) Polyclonal/MonoclonalAntibody, Unconjugated (ER1914-20) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Positive Control | Molt4 cell, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
