Application
Fig1: Sample: U937 Cell (Human) Lysate at 30 ug Primary: Anti- NUDCD3 (ER1914-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kDApplication
Fig2: Sample: Hl-60 Cell (Human) Lysate at 30 ug Primary: Anti- NUDCD3 (ER1914-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NUDCD3) Polyclonal Antibody, Unconjugated (ER1914-23) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | NUDCD3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA1068 NudC domain containing 3 NudC domain containing protein 3 NudC like protein NUDCD 3 NudCL OTTHUMP00000159457 NUDC3_HUMAN NudC domain-containing protein 3. . |
| Product description | NUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. NUDCD3 levels increase after the G1/S transition. |
| Immunogen | KLH conjugated synthetic peptide derived from human NUDCD3 161-260/361 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPig |
| Concentration | 1mg/ml |
| Alternative Names | KIAA1068 NudC domain containing 3 NudC domain containing protein 3 NudC like protein NUDCD 3 NudCL OTTHUMP00000159457 NUDC3_HUMAN NudC domain-containing protein 3. . |
|---|---|
| Molecular Weight(MW) | 41 KDa |
| SwissProt ID | Q8IVD9 |
|---|
Application
Fig1: Sample: U937 Cell (Human) Lysate at 30 ug Primary: Anti- NUDCD3 (ER1914-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kD
Application
Fig2: Sample: Hl-60 Cell (Human) Lysate at 30 ug Primary: Anti- NUDCD3 (ER1914-23) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NUDCD3) Polyclonal Antibody, Unconjugated (ER1914-23) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
