Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (ER1914-29) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Sample: Kidney(Mouse) lysate at 30ug; Primary: Anti-ODC(ER1914-29) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size:51 kD Observed band size:51 kDApplication
Fig3: Sample:Liver(Mouse) lysate at 30ug; Primary: Anti-ODC(ER1914-29) at 1:300 dilution; Secondary: AP conjugated Goat Anti-Rabbit IgG(bs-0295G-AP) at 1: 5000 dilution; Predicted band size : 51kD Observed band size : 51kDApplication
Fig4: Sample: Kidney(Mouse) lysate at 30ug; Primary: Anti-ODC(ER1914-29) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size:51 kD Observed band size:51 kDApplication
Fig5: Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (ER1914-29) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | ODC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ornithine decarboxylase 1 Ornithine decarboxylase Dodc1 Odc 1 ODC Odc1 ODC2 Ornithine decarboxylase 2 DCOR_HUMAN Ornithine decarboxylase 1 Ornithine decarboxylase Ornithine decarboxylase structural 1 RNODC. |
| Product description | SLC25A21 is a homolog of the S. cerevisiae ODC proteins, mitochondrial carriers that transport C5-C7 oxodicarboxylates across inner mitochondrial membranes. One of the species transported by ODC is 2-oxoadipate, a common intermediate in the catabolism of lysine, tryptophan, and hydroxylysine in mammals. Within mitochondria, 2-oxoadipate is converted into acetyl-CoA. |
| Immunogen | KLH conjugated synthetic peptide derived from human ODC 321-461/461 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Ornithine decarboxylase 1 Ornithine decarboxylase Dodc1 Odc 1 ODC Odc1 ODC2 Ornithine decarboxylase 2 DCOR_HUMAN Ornithine decarboxylase 1 Ornithine decarboxylase Ornithine decarboxylase structural 1 RNODC. |
|---|---|
| Molecular Weight(MW) | 51 KDa |
| Cellular Localization | Mitochondrion inner membrane |
| SwissProt ID | Q9BQT8 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (ER1914-29) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Sample: Kidney(Mouse) lysate at 30ug; Primary: Anti-ODC(ER1914-29) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size:51 kD Observed band size:51 kD
Application
Fig3: Sample:Liver(Mouse) lysate at 30ug; Primary: Anti-ODC(ER1914-29) at 1:300 dilution; Secondary: AP conjugated Goat Anti-Rabbit IgG(bs-0295G-AP) at 1: 5000 dilution; Predicted band size : 51kD Observed band size : 51kD
Application
Fig4: Sample: Kidney(Mouse) lysate at 30ug; Primary: Anti-ODC(ER1914-29) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size:51 kD Observed band size:51 kD
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (ER1914-29) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Rat stomach tissue, Mouse Kidney lysate, Mouse Liver lysate, Mouse Kidney lysate, rat liver tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
