Application
Fig1: Sample: Cerebral cortex (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Brain (Mouse) Lysate at 40 ug Brain (Rat) Lysate at 40 ug Primary: Anti- Orexin receptor 1+2 (ER1914-45) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (ER1914-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse Cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (ER1914-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Orexin receptor 1+2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Orexin Receptor 1 and 2 Orexin receptor 1 + 2 Orexin Receptor 2 HCRTR 1 HCRTR1 Hypocretin receptor type 1 Orexin receptor type 1 Ox-1-R Ox1-R Ox1r OX1R_HUMAN Hcrtr2 Hypocretin (orexin) receptor 2 Hypocretin receptor 2 Hypocretin receptor type 2 Orexin receptor 2 Orexin receptor type 2 Ox-2-R Ox2-R Ox2r OX2R_HUMAN. |
| Product description | The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A. Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. |
| Immunogen | KLH conjugated synthetic peptide derived from human Orexin receptor 1/2 321-425/444 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Orexin Receptor 1 and 2 Orexin receptor 1 + 2 Orexin Receptor 2 HCRTR 1 HCRTR1 Hypocretin receptor type 1 Orexin receptor type 1 Ox-1-R Ox1-R Ox1r OX1R_HUMAN Hcrtr2 Hypocretin (orexin) receptor 2 Hypocretin receptor 2 Hypocretin receptor type 2 Orexin receptor 2 Orexin receptor type 2 Ox-2-R Ox2-R Ox2r OX2R_HUMAN. |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | O43614 |
|---|
Application
Fig1: Sample: Cerebral cortex (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Brain (Mouse) Lysate at 40 ug Brain (Rat) Lysate at 40 ug Primary: Anti- Orexin receptor 1+2 (ER1914-45) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (ER1914-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse Cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (ER1914-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Rat brain tissue, Mouse Cerebral cortex Lysate, Mouse Hippocampus Lysate, Mouse Brain Lysate, Rat Brain Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
