Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse embryos); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Osterix) Polyclonal Antibody, Unconjugated (ER1914-47) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Embryo(Mouse) lysate at 30ug; Embryo(Mouse) lysate at 30ug; Primary: Anti-Osterix (ER1914-47) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 45kD Observed band size : 45kDApplication
Fig3: Sample:Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-Osterix(ER1914-47)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kD| Product Name | Osterix |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Osterix MGC126598 Osx Sp 7 Sp7 Sp7 transcription factor Transcription factor Sp7 Zinc finger protein osterix SP7_HUMAN. |
| Product description | Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human Osterix 331-431/431 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Osterix MGC126598 Osx Sp 7 Sp7 Sp7 transcription factor Transcription factor Sp7 Zinc finger protein osterix SP7_HUMAN. |
|---|---|
| Molecular Weight(MW) | 45 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q8TDD2 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse embryos); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Osterix) Polyclonal Antibody, Unconjugated (ER1914-47) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Embryo(Mouse) lysate at 30ug; Embryo(Mouse) lysate at 30ug; Primary: Anti-Osterix (ER1914-47) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 45kD Observed band size : 45kD
Application
Fig3: Sample:Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-Osterix(ER1914-47)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kD| Positive Control | Osteoblast/chondrocyte specific. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
