Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (OVOL2) Polyclonal Antibody, Unconjugated (ER1914-53) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Sample: Testis(Mouse) Cell Lysate at 40 ug Primary: Anti-OVOL2 (ER1914-53) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Product Name | OVOL2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bA504H3.3 EUROIMAGE566589 hOvo 2 hOvo2 Ovo like 2 (Drosophila) Ovo like 2 OVOL 2 Transcription factor Ovo like 2 Zinc finger protein 339 ZNF 339 ZNF339 OVOL2_HUMAN. |
| Product description | OVOL2 (Ovo like 2) contains 4 C2H2 type zinc fingers. It belongs to the krueppel C2H2 type zinc finger protein family. It is a DNA binding protein that binds to the 5'-G[GCT]GGGGG-3' core sequence. It probably acts as a transcription regulator. |
| Immunogen | KLH conjugated synthetic peptide derived from human OVOL2 75-180/275 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCow |
| Concentration | 1mg/ml |
| Alternative Names | bA504H3.3 EUROIMAGE566589 hOvo 2 hOvo2 Ovo like 2 (Drosophila) Ovo like 2 OVOL 2 Transcription factor Ovo like 2 Zinc finger protein 339 ZNF 339 ZNF339 OVOL2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 30 KDa |
| Cellular Localization | Nuclear. |
| SwissProt ID | Q9BRP0 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (OVOL2) Polyclonal Antibody, Unconjugated (ER1914-53) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Sample: Testis(Mouse) Cell Lysate at 40 ug Primary: Anti-OVOL2 (ER1914-53) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Positive Control | Expressed in testis, ovary, heart and skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
