Application
Fig1: Blank control: A431. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (ER1914-55) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Hcclm3(Human) Cell Lysate at 30 ug Primary: Anti-ox-LDL (ER1914-55) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated (ER1914-55) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | ox-LDL |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ox-LDL: LDL (Copper oxidized) Cu2SO4 oxidized low density lipoprotein oxidized low density lipoprotein Low density lipoprotein MDA oxidized LDL MDA oxidized low density lipoprotein. |
| Immunogen | Full length protein from human plasma |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ox-LDL: LDL (Copper oxidized) Cu2SO4 oxidized low density lipoprotein oxidized low density lipoprotein Low density lipoprotein MDA oxidized LDL MDA oxidized low density lipoprotein. |
|---|---|
| Molecular Weight(MW) | 31 KDa |
| Cellular Localization | Secreted (probable) |
Application
Fig1: Blank control: A431. Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (ER1914-55) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Hcclm3(Human) Cell Lysate at 30 ug Primary: Anti-ox-LDL (ER1914-55) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated (ER1914-55) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
