Application
Fig1: Protein: intestinal(mouse) lysate at 30ug; Raji(human) lysate at 30ug; Primary: rabbit Anti-p21-ARC (ER1914-57) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:20 kD Observed band size: 20 kDApplication
Fig2: Protein: intestinal(mouse) lysate at 30ug; Primary: rabbit Anti-p21-ARC (ER1914-57) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:20 kD Observed band size: 20 kDApplication
Fig3: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-p21-ARC (ER1914-57) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD| Product Name | p21-ARC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Actin related protein 2/3 complex subunit 3 ARC21 ARPC3_HUMAN ARP2/3 complex 21 kDa subunit ARP2/3 protein complex subunit p21 ARPC3. |
| Product description | p21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004). |
| Immunogen | KLH conjugated synthetic peptide derived from human p21-ARC 2-100/178 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenCowRabbitXenopus |
| Concentration | 1mg/ml |
| Alternative Names | Actin related protein 2/3 complex subunit 3 ARC21 ARPC3_HUMAN ARP2/3 complex 21 kDa subunit ARP2/3 protein complex subunit p21 ARPC3. |
|---|---|
| Molecular Weight(MW) | 20 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell projection. |
| SwissProt ID | O15145 |
|---|
Application
Fig1: Protein: intestinal(mouse) lysate at 30ug; Raji(human) lysate at 30ug; Primary: rabbit Anti-p21-ARC (ER1914-57) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:20 kD Observed band size: 20 kD
Application
Fig2: Protein: intestinal(mouse) lysate at 30ug; Primary: rabbit Anti-p21-ARC (ER1914-57) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:20 kD Observed band size: 20 kD
Application
Fig3: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-p21-ARC (ER1914-57) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
