Application
Fig1: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti-P2X2 (ER1914-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:52 kD Observed band size: 67 kDApplication
Fig2: Blank control:MCF7. Primary Antibody (green line): Rabbit Anti-P2X2 antibody (ER1914-58) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 2μg /test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | P2X2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP receptor P2RX 2 P2RX2 P2RX2_HUMAN P2X purinoceptor 2 P2X Receptor subunit 2 P2X2 P2X2a Purinergic receptor Purinergic receptor P2X ligand gated ion channel 2 Purinergic receptor P2X2 isoform A Purinergic receptor P2X2 isoform B Purinergic receptor P2X2 isoform C Purinergic receptor P2X2 isoform D Purinergic receptor P2X2 isoform H Purinergic receptor P2X2 isoform I MGC129601. |
| Product description | Binding of this ligand-gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. |
| Immunogen | KLH conjugated synthetic peptide derived from human ATP receptor 251-350/471 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:2ug/test: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | ATP receptor P2RX 2 P2RX2 P2RX2_HUMAN P2X purinoceptor 2 P2X Receptor subunit 2 P2X2 P2X2a Purinergic receptor Purinergic receptor P2X ligand gated ion channel 2 Purinergic receptor P2X2 isoform A Purinergic receptor P2X2 isoform B Purinergic receptor P2X2 isoform C Purinergic receptor P2X2 isoform D Purinergic receptor P2X2 isoform H Purinergic receptor P2X2 isoform I MGC129601. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | Q9UBL9 |
|---|
Application
Fig1: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti-P2X2 (ER1914-58) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:52 kD Observed band size: 67 kD
Application
Fig2: Blank control:MCF7. Primary Antibody (green line): Rabbit Anti-P2X2 antibody (ER1914-58) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 2μg /test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Application Notes | WB:1:500-2000: FC:2ug/test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
