Application
Fig1: Sample: Raji Cell (Human) Lysate at 30 ug Primary: Anti- P2Y8 (ER1914-62) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 50 kDApplication
Fig2: Blank control: HL60. Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (ER1914-62) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (blue line): Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig3: Blank control: Raji. Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (ER1914-62) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | P2Y8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P2RY8 P2RY8_HUMAN P2Y purinoceptor 8 P2Y8 Purinergic receptor P2Y G protein coupled 8. |
| Product description | Probable receptor for purines coupled to G-proteins. |
| Immunogen | KLH conjugated synthetic peptide derived from human P2Y8 131-230/359 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:2ug/Test: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | P2RY8 P2RY8_HUMAN P2Y purinoceptor 8 P2Y8 Purinergic receptor P2Y G protein coupled 8. |
|---|---|
| Molecular Weight(MW) | 41 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q86VZ1 |
|---|
Application
Fig1: Sample: Raji Cell (Human) Lysate at 30 ug Primary: Anti- P2Y8 (ER1914-62) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 50 kD
Application
Fig2: Blank control: HL60. Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (ER1914-62) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (blue line): Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig3: Blank control: Raji. Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (ER1914-62) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Barely detectable in normal blood leukocytes. Weaker expression was seen in heart, kidney and lung. Not detected in brain. |
|---|---|
| Application Notes | WB:1:500-2000: FC:2ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
