Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (ER1914-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: k562 (human)cell Lysate at 40 ug Primary: Anti- P2Y9 (ER1914-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 48 kDApplication
Fig3: Sample: Brain (mouse) Lysate at 40 ug Primary: Anti- P2Y9 (ER1914-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 48 kDApplication
Fig4: Sample: HL-60 Cell (Human) Lysate at 30 ug Primary: Anti-P2Y9 (ER1914-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig5: Sample: Cerebrum (Mouse) Lysate at 40 ug Ovary (Mouse) Lysate at 40 ug Primary: Anti-P2Y9 (ER1914-63) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kDApplication
Fig6: Sample: HL-60(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti-P2Y9 (ER1914-63) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kD| Product Name | P2Y9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5730485F04Rik G protein coupled receptor 23 G-protein coupled receptor 23 GPR23 LPA receptor 4 LPA-4 LPA4 Lpar4 LPAR4_HUMAN Lysophosphatidic acid receptor 4 Lysophosphatidic Acid Receptor P2Y9 P2RY9 P2Y purinoceptor 9 P2Y5 like P2Y5-like receptor P2Y9 Purinergic receptor 9. |
| Product description | Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA. |
| Immunogen | KLH conjugated synthetic peptide derived from human P2Y9 175-270/370 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | 5730485F04Rik G protein coupled receptor 23 G-protein coupled receptor 23 GPR23 LPA receptor 4 LPA-4 LPA4 Lpar4 LPAR4_HUMAN Lysophosphatidic acid receptor 4 Lysophosphatidic Acid Receptor P2Y9 P2RY9 P2Y purinoceptor 9 P2Y5 like P2Y5-like receptor P2Y9 Purinergic receptor 9. |
|---|---|
| Molecular Weight(MW) | 42 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q8BLG2 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (ER1914-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: k562 (human)cell Lysate at 40 ug Primary: Anti- P2Y9 (ER1914-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 48 kD
Application
Fig3: Sample: Brain (mouse) Lysate at 40 ug Primary: Anti- P2Y9 (ER1914-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 48 kD
Application
Fig4: Sample: HL-60 Cell (Human) Lysate at 30 ug Primary: Anti-P2Y9 (ER1914-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig5: Sample: Cerebrum (Mouse) Lysate at 40 ug Ovary (Mouse) Lysate at 40 ug Primary: Anti-P2Y9 (ER1914-63) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kD
Application
Fig6: Sample: HL-60(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti-P2Y9 (ER1914-63) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kD| Positive Control | High expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
