Application
Fig1: Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-P450 1A2 antibody (ER1914-65) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 0.2μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-P450 1A2 (ER1914-65) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD| Product Name | P450 1A2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYP1A2 Cytochrome P450 1A2 cytochrome P450, family 1, subfamily a, polypeptide 2 Cytochrome P450 family 1 polypeptide 2 Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 Cyp1a2 CYPD45 P-450d RATCYPD45 CYP1A2 CP12 CP 12 P3-450 Aryl hydrocarbon hydroxylase Dioxin inducable P3 45 Flavoprotein linked monooxygenase Microsomal monooxygenase P450 4 P450 form 4 P450 P3 P450(PA) Xenobiotic monooxygenase. |
| Product description | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. |
| Immunogen | KLH conjugated synthetic peptide derived from human P450 1A2/CYP1A2 221-320/513 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:0.2ug/test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CYP1A2
Cytochrome P450 1A2
cytochrome P450 family 1 subfamily a polypeptide 2 Cytochrome P450 family 1 polypeptide 2 Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 Cyp1a2 CYPD45 P-450d RATCYPD45 CYP1A2 CP12 CP 12 P3-450 Aryl hydrocarbon hydroxylase Dioxin inducable P3 45 Flavoprotein linked monooxygenase Microsomal monooxygenase P450 4 P450 form 4 P450 P3 P450(PA) Xenobiotic monooxygenase. |
|---|---|
| Molecular Weight(MW) | 56 KDa |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| SwissProt ID | P05177 |
|---|
Application
Fig1: Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-P450 1A2 antibody (ER1914-65) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 0.2μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-P450 1A2 (ER1914-65) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD| Positive Control | Liver. |
|---|---|
| Application Notes | WB:1:500-2000: FC:0.2ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
