Application
Fig1: Sample: bone (Mouse) Lysate at 40 ug Primary: Anti-PAG1(ER1914-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 45 kD| Product Name | PAG1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBP Csk Binding Protein Csk-binding protein FLJ37858 MGC138364 PAG 1 PAG PAG1 PAG1_HUMAN Phosphoprotein associated with glycosphingolipid enriched microdomains 1 Phosphoprotein associated with glycosphingolipid enriched microdomains Phosphoprotein associated with glycosphingolipid microdomains 1 Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 Protein Associated with Glycosphingolipid Enriched Microdomains Transmembrane adapter protein PAG Transmembrane phosphoprotein Cbp. |
| Product description | Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. |
| Immunogen | KLH conjugated synthetic peptide derived from human PAG1 331-432/432 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | CBP Csk Binding Protein Csk-binding protein FLJ37858 MGC138364 PAG 1 PAG PAG1 PAG1_HUMAN Phosphoprotein associated with glycosphingolipid enriched microdomains 1 Phosphoprotein associated with glycosphingolipid enriched microdomains Phosphoprotein associated with glycosphingolipid microdomains 1 Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 Protein Associated with Glycosphingolipid Enriched Microdomains Transmembrane adapter protein PAG Transmembrane phosphoprotein Cbp. |
|---|---|
| Molecular Weight(MW) | 47 KDa |
| Cellular Localization | Cell membrane. Present in lipid rafts. |
| SwissProt ID | Q9NWQ8 |
|---|
Application
Fig1: Sample: bone (Mouse) Lysate at 40 ug Primary: Anti-PAG1(ER1914-70) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 45 kD| Positive Control | Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
