Application
Fig1: Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).Application
Fig2: Sample: HepG2(Human) Cell Lysate at 40 ug Primary: Anti-PALB2 (ER1914-72) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kDApplication
Fig3: Sample: MDA-MB-231(Human) Cell Lysate at 40 ug Primary: Anti-PALB2 (ER1914-72) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD| Product Name | PALB2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp667I166 4732427B05 BC066140 DKFZp686E1054 FANCN FANCN GENE FLJ21816 OTTMUSP00000025884 PALB2 partner and localizer of BRCA2 RGD1304759 PALB2_HUMAN. |
| Product description | Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. |
| Immunogen | KLH conjugated synthetic peptide derived from human PALB2 1101-1186/1186 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatPigCow |
| Concentration | 1mg/ml |
| Alternative Names | DKFZp667I166 4732427B05 BC066140 DKFZp686E1054 FANCN FANCN GENE FLJ21816 OTTMUSP00000025884 PALB2 partner and localizer of BRCA2 RGD1304759 PALB2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 131 KDa |
| Cellular Localization | Nucleus. Note=Colocalizes with BRCA2 in nuclear foci. |
| SwissProt ID | Q86YC2 |
|---|
Application
Fig1: Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
Application
Fig2: Sample: HepG2(Human) Cell Lysate at 40 ug Primary: Anti-PALB2 (ER1914-72) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD
Application
Fig3: Sample: MDA-MB-231(Human) Cell Lysate at 40 ug Primary: Anti-PALB2 (ER1914-72) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
