Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-Pancreatic Amylase (ER1914-75) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kDApplication
ogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pancreatic Amylase) Polyclonal Antibody, Unconjugated (ER1914-75) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-Pancreatic Amylase (ER1914-75) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 61 kD| Product Name | Pancreatic Amylase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha amylase Alpha amylase pancreatic precursor AMY2 AMY2A AMY2B Amylase alpha 2A Amylase alpha 2A pancreatic Amylase alpha 2B Amylase alpha 2B pancreatic Amylase pancreatic alpha 2A Amylase pancreatic alpha 2B Pancreatic alpha amylase AMYP_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from human Pancreatic Amylase 151-250/511 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Alpha amylase Alpha amylase pancreatic precursor AMY2 AMY2A AMY2B Amylase alpha 2A Amylase alpha 2A pancreatic Amylase alpha 2B Amylase alpha 2B pancreatic Amylase pancreatic alpha 2A Amylase pancreatic alpha 2B Pancreatic alpha amylase AMYP_HUMAN. |
|---|---|
| Molecular Weight(MW) | 56 KDa |
| Cellular Localization | Secreted, extracellular space. |
| SwissProt ID | P04746 |
|---|
Application
Fig1: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-Pancreatic Amylase (ER1914-75) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD
Application
ogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Pancreatic Amylase) Polyclonal Antibody, Unconjugated (ER1914-75) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-Pancreatic Amylase (ER1914-75) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 61 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
