Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-PAP2B (ER1914-76) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 35 kDApplication
Fig2: Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti-PAP2B (ER1914-76) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 35 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PAP2B) Polyclonal Antibody, Unconjugated (ER1914-76) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | PAP2B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dri42 Lipid phosphate phosphohydrolase 3 LPP3 PAP 2b PAP2 beta PAP2b Vascular endothelial growth factor and type I collagen inducible protein VCIP. |
| Product description | Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA = PA > C-1-P > S-1-P. May be involved in cell adhesion and in cell-cell interactions. |
| Immunogen | KLH conjugated synthetic peptide derived from human PAP2B 241-311/311 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Dri42 Lipid phosphate phosphohydrolase 3 LPP3 PAP 2b PAP2 beta PAP2b Vascular endothelial growth factor and type I collagen inducible protein VCIP. |
|---|---|
| Molecular Weight(MW) | 34 KDa |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | O14495 |
|---|
Application
Fig1: Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-PAP2B (ER1914-76) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 35 kD
Application
Fig2: Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti-PAP2B (ER1914-76) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 35 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PAP2B) Polyclonal Antibody, Unconjugated (ER1914-76) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Ubiquitously expressed. Highly expressed in heart and placenta. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
