Application
Fig1: Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug Primary: Anti-PCDH20 (ER1914-83) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 98 kDApplication
Fig2: Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- PCDH20 antibody(ER1914-83), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig3: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-PCDH20 (ER1914-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 100 kDApplication
Fig4: Sample: Hippocampus (Mouse) Lysate at 40 ug Olfactory bulb (Mouse) Lysate at 40 ug Primary: Anti-PCDH20 (ER1914-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 100 kD| Product Name | PCDH20 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCD20_HUMAN PCDH 13 PCDH 20 PCDH13 PCDH20 Protocadherin 13 Protocadherin 20 Protocadherin-13 Protocadherin-20 Protocadherin13 Protocadherin20. |
| Product description | Potential calcium-dependent cell-adhesion protein. |
| Immunogen | KLH conjugated synthetic peptide derived from human PCDH20 611-710/924 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | PCD20_HUMAN PCDH 13 PCDH 20 PCDH13 PCDH20 Protocadherin 13 Protocadherin 20 Protocadherin-13 Protocadherin-20 Protocadherin13 Protocadherin20. |
|---|---|
| Molecular Weight(MW) | 98 KDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q8N6Y1 |
|---|
Application
Fig1: Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug Primary: Anti-PCDH20 (ER1914-83) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 98 kD
Application
Fig2: Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- PCDH20 antibody(ER1914-83), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig3: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-PCDH20 (ER1914-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 100 kD
Application
Fig4: Sample: Hippocampus (Mouse) Lysate at 40 ug Olfactory bulb (Mouse) Lysate at 40 ug Primary: Anti-PCDH20 (ER1914-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 100 kD| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
